Floating disks correspond to the photosynthetic rate because when more disks float it shows that the photosynthesis is occurring. Also, if the disks rise faster it shows that photosynthesis is occurring faster. If the disks do not float it shows that photosynthesis is not happening or that is happening very slowly.
Did any leaf disks float in the dark treatment?
When you put floating leaf disks in the dark, they will eventually sink. Without light energy, no photosynthesis will occur, so no more O2 gas will be produced. However, respiration continues in the dark, so the disks will use the accumulated O2 gas.
Why did the leaf disks float?
Bicarbonate ion serves as the carbon source for photosynthesis. As photosynthesis proceeds oxygen is released into the interior of the leaf which changes the buoyancy–causing the disks to rise.
Why did the spinach disks float?
When exposed to light, the disks use carbon dioxide and water to produce oxygen and glucose. Oxygen released from the leaves forms tiny bubbles that cause the leaves to float.
What was the ET50?
The ET50 is the time it takes for 50% of the leaf disks to float and is a good indicator of the rate of photosynthesis. It was hypothesized that if the concentration of carbon dioxide was increased, then the rate at which photosynthesis occurs will also increase.
Why did the leaf discs float during the photosynthesis experiment?
The light source provides light energy, the solution provides water, and sodium bicarbonate provides dissolved CO2. Plant material will generally float in water. This is because leaves have air in the spaces between cells, which helps them collect CO2 gas from their environment to use in photosynthesis.
How can floating leaf disks determine the rate of photosynthesis?
As photosynthesis proceeds, oxygen is released into the interior of the leaf, which changes the buoyancy- causing the disks to rise. Since cellular respiration is taking place at the same time and consuming oxygen, the rate that the disks rise is an indirect measurement of the net rate of photosynthesis.
What causes leaf disks to float and/or sink?
Leaf disks normally float, however when the air spaces are infiltrated with carbon dioxide, the overall density of the lead disk increases and the leaf disk sinks. When sodium bicarbonate is added to the water, the bicarbonate ion acts as a carbon source for photosynthesis causing the leaf disks to sink.
What is the floating leaf experiment?
In the floating leaf disk assay, 10 or more leaf disk samples are punched out of a leaf. In the next step, a vacuum is used to replace the air pockets within the leaf structure with a baking soda (bicarbonate) solution. The baking soda provides the carbon dioxide that the leaf needs for photosynthesis.
What causes the leaf disks to float quizlet?
The bicarbonate ion serves as the carbon source for photosynthesis. Then, as photosynthesis continues, oxygen is released into the leaf, which causes the leaf disk to rise.
How does the floating disk method measure photosynthesis?
As photosynthesis proceeds oxygen is released into the interior of the leaf which changes the buoyancy–causing the disks to rise. Since cellular respiration is taking place at the same time, consuming oxygen, the rate that the disks rise is an indirect measurement of the net rate of photosynthesis.
Why does a floating disk indicate that photosynthesis is occurring?
Because the leaf disks are living plant material that still require energy, they are simultaneously using O2 gas during respiration and producing O2 gas during photosynthesis. Therefore, the bubbles of O2 that you see represent the net products of photosynthesis, minus the O2 used by respiration.
How do leaf discs investigate photosynthesis?
Students punch out small discs from leaves, and float them in a syringe of sodium hydrogen carbonate solution. Once gas is evolved by photosynthesis, the leaf discs rise and fall. Students can compare the rate of photosynthesis in sun and shade plants and at different light intensities, amongst many other factors.
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5 Were there any treatments syringes without floating disks The four …
The four disks that were placed in the dark showed no signs of change and all leaves remained at the bottom. 6. How do floating disks correspond to the rate of photosynthesis? Floating disks correspond to the rate of photosynthesis because however long it takes for the disks to float is how long it takes for photosynthesis to occur. 7.
6 Were there any treatments syringes without floating disks Yes the …
6 Were there any treatments syringes without floating disks Yes the dark from BIO 110 at Davidson County Community College. Study Resources. Main Menu; by School; by Literature Title; … 6 Were there any treatments syringes without floating disks Yes the dark. 6 were there any treatments syringes without floating.
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Syringes C and D with the 0% solution of Baking Soda had the most floating leaf disks after 35 minutes. Were there any treatments (syringes) without floating disks? Syringes A and B with 0% Baking Soda in Both Light and Dark that didn’t have any floating disks. How do floating disks correspond to the rate of photosynthesis? The floating leaf …
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Infiltrate the leaf disks with sodium bicarbonate solution. Remove the plunger of the syringe and place the leaf disks into the syringe barrel. Replace the plunger being careful not to crush the leaf disks. Push on the plunger until only a small volume of air and leaf disk remain in the barrel (Floating Spinach Disks Photosynthesis Demonstration
Dip the syringe in the baking soda/ detergent solution and draw in about 3 ccs of liquid. Tap the syringe to suspend the leaves in the solution. Push the plunger to expel excess air, then place your finger over the end of the syringe and pull back on the plunger to create a vacuum. While maintaining the vacuum, swirl the leaf disks in the syringe.
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10-mL syringe (without a needle) Clear plastic cup (1-cup size) or 250-mL beaker … Remove the plunger from the syringe, and remove the cover from the tip, if there is one. Put the leaf disks into the barrel of the syringe, and tap them down to the tip. … the number traditionally used is the time point at which half of the disks in the …
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Which syringe had the most leaf disks floating after 20 minutes? Results may vary. The syringe with a baking soda solution and bright light should have the most leaf disks floating after 20 minutes. 8. Were there any syringes without floating disks? Results may vary. The syringes without a light source should not have floating leaf disks. 9.
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Resource
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